Biology

Characterization of IS 1676 from Rhodococcus erythropolis SQ1

Document Type

Article

Abstract

To develop a transposable element-based system for mutagenesis in Rhodococcus, we used the sacB gene from Bacillus subtilis to isolate a novel transposable element, IS1676, from R. erythropolis SQ1. This 1693 bp insertion sequence is bounded by imperfect (10 out of 13 bp) inverted repeats and it creates 4 bp direct repeats upon insertion. Comparison of multiple insertion sites reveals a preference for the sequence 5'-(C/T)TA(A/G)-3' in the target site. IS1676 contains a single, large (1446 bp) open reading frame with coding potential for a protein of 482 amino acids. IS1676 may be similar to an ancestral transposable element that gave rise to repetitive sequences identified in clinical isolates of Mycobacterium kansasii. Derivatives of IS1676 should be useful for analysis of Rhodococcus strains, for which few other genetic tools are currently available.

Publication Title

Applied Microbiology and Biotechnology

Publication Date

1999

Volume

52

Issue

6

First Page

811

Last Page

819

ISSN

0175-7598

DOI

10.1007/s002530051597

Keywords

Rhodococcus

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