Biology
Document Type
Article
Abstract
Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.
This article has 113 total authors; only 32 are listed here.
Publication Title
Nature Methods
Publication Date
5-1-2020
Volume
17
Issue
5
First Page
481
Last Page
494
ISSN
1548-7091
DOI
10.1038/s41592-020-0796-x
Keywords
biodiversity; DNA, ecosystem, environment, Eukaryota, green fluorescent proteins, marine biology, models, biological, species specificity, transformation, genetic
Repository Citation
Faktorová, Drahomíra; Nisbet, R. Ellen R.; Fernández Robledo, José A.; Casacuberta, Elena; Sudek, Lisa; Allen, Andrew E.; Ares, Manuel; Aresté, Cristina; Balestreri, Cecilia; Barbrook, Adrian C.; Beardslee, Patrick; Bender, Sara; Booth, David S.; Bouget, François Yves; Bowler, Chris; Breglia, Susana A.; Brownlee, Colin; Burger, Gertraud; Cerutti, Heriberto; Cesaroni, Rachele; Chiurillo, Miguel A.; Clemente, Thomas; Coles, Duncan B.; Collier, Jackie L.; Cooney, Elizabeth C.; Coyne, Kathryn; Docampo, Roberto; Dupont, Christopher L.; Edgcomb, Virginia; Einarsson, Elin; Elustondo, Pía A.; and Robertson, Deborah, "Genetic tool development in marine protists: emerging model organisms for experimental cell biology" (2020). Biology. 29.
https://commons.clarku.edu/faculty_biology/29
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright Conditions
Published source must be acknowledged with citation: Faktorová, D., Nisbet, R. E. R., Fernández Robledo, J. A., Casacuberta, E., Sudek, L., Allen, A. E., ... & Lukeš, J. (2020). Genetic tool development in marine protists: emerging model organisms for experimental cell biology. Nature Methods, 17(5), 481-494.
