Biology
A silencer element identified in Drosophila is required for imprinting of H19 reporter transgenes in mice
Document Type
Article
Abstract
The H19 gene is subject to genomic imprinting because it is methylated and repressed after paternal inheritance and is unmethylated and expressed after maternal inheritance. We recently identified a 1.1-kb control element in the upstream region of the H19 gene that functions as a cis-acting silencer element in Drosophila. Here we investigate the function of this element in mice. We demonstrate that both H19-lacZ and H19-PLAP reporter transgenes can undergo imprinting with repression and hypermethylation after paternal transmission at many integration sites. However, transgenes that were deleted for the 1.1-kb silencer element showed loss of paternal repression, but they did not show marked changes in the paternal methylation of the remaining upstream region. This study demonstrates that the 1.1-kb control element identified in Drosophila is required to silence paternally transmitted H19 minitransgenes in mice.
Publication Title
Proceedings of the National Academy of Sciences of the United States of America
Publication Date
8-3-1999
Volume
96
Issue
16
First Page
9242
Last Page
9247
ISSN
0027-8424
DOI
10.1073/pnas.96.16.9242
Keywords
Drosophila, silencer, mice, genomic imprinting
Repository Citation
Brenton, J. D.; Drewell, R. A.; Viville, S.; Hilton, K. J.; Barton, S. C.; Ainscough, J. F.X.; and Surani, M. A., "A silencer element identified in Drosophila is required for imprinting of H19 reporter transgenes in mice" (1999). Biology. 161.
https://commons.clarku.edu/faculty_biology/161
