Inhibition and Overexpression Studies of Lysyl Oxidase
Date of Award
Master of Science in Biochemistry & Molecular Biology
Charles E. Jakobsche
Frederick T. Greenaway
Donald E. Spratt
Lysyl oxidase (LOX) is a 29 kDa quinone-dependent amine oxidase that catalyzes crosslinking reactions in collagen and elastin in developing extracellular matrix. LOX is a current target of medical research due to its relevance in metastatic tumor progression. LOX is a difficult target to study because current attempts at recombinant expression and purification from natural sources are low yielding, and LOX has low solubility in most aqueous buffers. Hydrazine-containing compounds are known inhibitors of LOX and other quinone-dependent amine oxidases; therefore three novel small molecules and two activity- based molecular probes incorporating the hydrazine functionality were synthesized and tested.
Burke, Ashley A., "Inhibition and Overexpression Studies of Lysyl Oxidase" (2017). Biology. 12.