Biology
Document Type
Article
Abstract
Coordination between growth and patterning/differentiation is critical if appropriate final organ structure and size is to be achieved. Understanding how these two processes are regulated is therefore a fundamental and as yet incompletely answered question. Here we show through genetic analysis that the phospholipase C-γ (PLC-γ) encoded by small wing (sl) acts as such a link between growth and patterning/differentiation by modulating some MAPK outputs once activated by the insulin pathway; particularly, sl promotes growth and suppresses ectopic differentiation in the developing eye and wing, allowing cells to attain a normal size and differentiate properly. sl mutants have previously been shown to have a combination of both growth and patterning/differentiation phenotypes: small wings, ectopic wing veins, and extra R7 photoreceptor cells. We show here that PLC-γ activated by the insulin pathway participates broadly and positively during cell growth modulating EGF pathway activity, whereas in cell differentiation PLC-γ activated by the insulin receptor negatively regulates the EGF pathway. These roles require different SH2 domains of PLC-γ, and act via classic PLC-γ signaling and EGF ligand processing. By means of PLC-γ, the insulin receptor therefore modulates differentiation as well as growth. Overall, our results provide evidence that PLC-γ acts during development at a time when growth ends and differentiation begins, and is important for proper coordination of these two processes. © 2011 Murillo-Maldonado et al.
Publication Title
PLoS ONE
Publication Date
11-21-2011
Volume
6
Issue
11
ISSN
1932-6203
DOI
10.1371/journal.pone.0028067
Keywords
cell differentiation, cell growth
Repository Citation
Murillo-Maldonado, Juan M.; Bou Zeineddine, Fouad; Stock, Rachel; Thackeray, Justin; and Riesgo-Escovar, Juan R., "Insulin receptor-mediated signaling via phospholipase C-γ regulates growth and differentiation in drosophila" (2011). Biology. 395.
https://commons.clarku.edu/faculty_biology/395
Cross Post Location
Student Publications
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright Conditions
Murillo-Maldonado, J. M., Zeineddine, F. B., Stock, R., Thackeray, J., & Riesgo-Escovar, J. R. (2011). Insulin receptor-mediated signaling via phospholipase C-γ regulates growth and differentiation in Drosophila. PLoS One, 6(11), e28067. https://doi.org/10.1371/journal.pone.0028067
