Biology

A user's guide to restriction enzyme-mediated integration in Dictyostelium

Document Type

Article

Abstract

Restriction enzyme-mediated integration (REMI) has been used to study a number of cellular and developmental processes in Dictyostelium discoideum. In this paper we review the basics of this powerful method of introducing random mutations in Dictyostelium. Here we discuss several mutation screens that have been devised and some of the genes that have been discovered through this approach to mutagenesis. Included in this discussion is how one goes about isolating a gene that has been disrupted by REMI, and how one confirms that this disruption is actually responsible for the observed phenotype. Finally, we describe how REMI can be used as an effective teaching tool in undergraduate cell biology laboratory courses.

Publication Title

Journal of Muscle Research and Cell Motility

Publication Date

12-2002

Volume

23

Issue

7-8

First Page

597

Last Page

604

ISSN

0142-4319

DOI

10.1023/A:1024494704863

Keywords

Restriction enzyme-mediated integration, Dictyostelium discoideum

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