Chemistry

Fluorescent Sensors of Phosphate Containing Biomolecules

Document Type

Article

Abstract

Phosphates are an essential functional group in living organisms. Phosphates are required to synthesize ATP, the energy unit in cells, and the synthesis of DNA and RNA, phosphoproteins, phospholipids, and various metabolites. This article is focused on reviewing current research on fluorescent sensing of biologically relevant phosphate functionalities in an aqueous environment or more complex environments such as live cells. There are three different approaches for the fluorescent detection of phosphates. These involve using metal-ligand complexes as sensors, using metal-free small molecules as sensors, and using protein or peptide molecules as sensors. The utilization of these approaches for fluorescent sensing of different phosphate-containing biomolecule classes such as nucleoside polyphosphates, phosphorylated peptides, phosphorylated proteins, pyrophosphates, phospholipids, and RNA are discussed.

Publication Title

Israel Journal of Chemistry

Publication Date

3-2021

Volume

61

Issue

3-4

First Page

169

Last Page

184

ISSN

0021-2148

DOI

10.1002/ijch.202000087

Keywords

biosensing, fluorescent indicators, metal complex, microscopy, phosphorylation

Link to Full Text

Share

COinS