Chemistry
Document Type
Article
Abstract
ITCH (aka Atrophin-1-interacting protein 4) is a prominent member of the NEDD4 HECT (Homologous to E6AP C-Terminus) E3 ubiquitin ligase family that regulates numerous cellular functions including inflammatory responses through T-cell activation, cell differentiation, and apoptosis. Known intracellular targets of ITCH-dependent ubiquitylation include receptor proteins, signaling molecules, and transcription factors. The HECT C-terminal lobe of ITCH contains the conserved catalytic cysteine required for the covalent attachment of ubiquitin onto a substrate and polyubiquitin chain assembly. We report here the complete experimentally determined 1 H, 13 C, and 15 N backbone and sidechain resonance assignments for the HECT C-terminal lobe of ITCH (residues 784–903) using heteronuclear, multidimensional NMR spectroscopy. These resonance assignments will be used in future NMR-based studies to examine the role of dynamics and conformational flexibility in HECT-dependent ubiquitylation as well as deciphering the structural and biochemical basis for polyubiquitin chain synthesis and specificity by ITCH.
Publication Title
Biomolecular NMR Assignments
Publication Date
4-2019
Volume
13
Issue
1
First Page
15
Last Page
20
ISSN
1874-2718
DOI
10.1007/s12104-018-9843-2
Keywords
Atrophin-1-interacting protein 4, E3 ubiquitin ligase, HECT, ITCH, NMR spectroscopy, Ubiquitin, Ubiquitylation
Repository Citation
Beasley, Steven A.; Bardhi, Roela; and Spratt, Donald E., "1 H, 13 C, and 15 N resonance assignments of the C-terminal lobe of the human HECT E3 ubiquitin ligase ITCH" (2019). Chemistry. 150.
https://commons.clarku.edu/chemistry/150
Cross Post Location
Student Publications
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright Conditions
Beasley, S. A., Bardhi, R., & Spratt, D. E. (2019). 1 H, 13 C, and 15 N resonance assignments of the C-terminal lobe of the human HECT E3 ubiquitin ligase ITCH. Biomolecular NMR Assignments, 13, 15-20.
