Chemistry
Document Type
Article
Abstract
The insulin-degrading enzyme (IDE) possesses a strong ability to degrade insulin and Aβ42 that has been linked to the neurodegeneration in Alzheimer’s disease (AD). Given this, an attractive IDE-centric strategy for the development of therapeutics for AD is to boost IDE’s activity for the clearance of Aβ42 without offsetting insulin proteostasis. Recently, we showed that resveratrol enhances IDE’s activity toward Aβ42. In this work, we used a combination of chromatographic and spectroscopic techniques to investigate the effects of resveratrol on IDE’s activity toward insulin. For comparison, we also studied epigallocatechin-3-gallate (EGCG). Our results show that the two polyphenols affect the IDE-dependent degradation of insulin in different ways: EGCG inhibits IDE while resveratrol has no effect. These findings suggest that polyphenols provide a path for developing therapeutic strategies that can selectively target IDE substrate specificity.
Publication Title
Antioxidants
Publication Date
9-2021
Volume
10
Issue
9
ISSN
0146-3721
DOI
10.3390/antiox10091342
Keywords
Epigallocatechin-3-gallate, insulin-degrading enzyme, polyphenols, resveratrol
Repository Citation
Zheng, Qiuchen; Kebede, Micheal T.; Lee, Bethany; Krasinski, Claire A.; Islam, Saadman; Wurfl, Liliana A.; Kemeh, Merc M.; Ivancic, Valerie A.; Jakobsche, Charles E.; Spratt, Donald E.; and Lazo, Noel, "Differential effects of polyphenols on insulin proteolysis by the insulin-degrading enzyme" (2021). Chemistry. 111.
https://commons.clarku.edu/chemistry/111
Cross Post Location
Student Publications
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright Conditions
Zheng, Q., Kebede, M. T., Lee, B., Krasinski, C. A., Islam, S., Wurfl, L. A., ... & Lazo, N. D. (2021). Differential effects of polyphenols on insulin proteolysis by the insulin-degrading enzyme. Antioxidants, 10(9), 1342.
