Quantitative UV Sensitivity Assay and Whole Genome Extraction for DNA Methylation Analysis in Dictyostelium discoideum
Date of Award
2017
Degree Type
Thesis
Degree Name
Master of Science in Biology
Department
Biology
Chief Instructor
Denis A. Larochelle
Second Reader
Robert Drewell
Third Reader
John Gibbons
Keywords
Cellular biology
Abstract
Dictyostelium discoideum is an early diverging, social amoeba that demonstrates significant genomic similarity to humans, allowing for homologous study of a variety of biological features, such a proteins and genomic DNA. The proteins DdCenB and DdRad4 are both involved in DNA repair, and knockout cell lines for these proteins demonstrated significantly more UV sensitivity when compared to AX3 wildtype cells. CenB Mutant cells, mutated to inhibit interaction of DdRad4 and DdCenB, demonstrated similar UV sensitivity to the knockout cells. Overall, this study provided significant refinement to a previously developed 2D-bioprinter UV sensitivity assay, allowing for a greater degree of quantitative analysis and reproducibility.
Recommended Citation
James, Denis, "Quantitative UV Sensitivity Assay and Whole Genome Extraction for DNA Methylation Analysis in Dictyostelium discoideum" (2017). Biology. 7.
https://commons.clarku.edu/biology_masters_papers/7