Biology

Title

Quantitative UV Sensitivity Assay and Whole Genome Extraction for DNA Methylation Analysis in Dictyostelium discoideum

Date of Award

2017

Degree Type

Thesis

Degree Name

Master of Science in Biology

Department

Biology

Chief Instructor

Denis A. Larochelle

Second Reader

Robert Drewell

Third Reader

John Gibbons

Abstract

Dictyostelium discoideum is an early diverging, social amoeba that demonstrates significant genomic similarity to humans, allowing for homologous study of a variety of biological features, such a proteins and genomic DNA. The proteins DdCenB and DdRad4 are both involved in DNA repair, and knockout cell lines for these proteins demonstrated significantly more UV sensitivity when compared to AX3 wildtype cells. CenB Mutant cells, mutated to inhibit interaction of DdRad4 and DdCenB, demonstrated similar UV sensitivity to the knockout cells. Overall, this study provided significant refinement to a previously developed 2D-bioprinter UV sensitivity assay, allowing for a greater degree of quantitative analysis and reproducibility.

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