Modifying Amine Oxidases: Molecular Probes and High-Throughput Screening
Date of Award
Master of Science in Biochemistry & Molecular Biology
Charles E. Jakobsche
Noel D. Lazo
Donald E. Spratt
Amine oxidases are an essential and disease-relevant family of enzymes in the body. As the first of two overarching projects, a molecular probe was developed for flavin-dependent amine oxidases (Chapter 1). The flavin-dependent subfamily regulates crucial neurotransmitters including dopamine and epinephrine. Implicated in complex disorders such as depression, autism, and Parkinson's disease, a probe for the subfamily would enable the discovery of new life-improving drugs. Taking inspiration from known small molecular inhibitors, we designed and synthesized an activity-based probe, equipped with a propargylamine binding domain and a dinitrophenol readout domain. Using L-Amino Acid Oxidase as a model enzyme, a fluorescent-based activity assay was optimized, allowing measurement of inhibition.
Wall, Alexander Daniel, "Modifying Amine Oxidases: Molecular Probes and High-Throughput Screening" (2017). Biology. 5.